sgrna libraries Search Results


guide ittm assay  (TaKaRa)
93
TaKaRa guide ittm assay
Guide Ittm Assay, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
guide ittm assay - by Bioz Stars, 2026-04
93/100 stars
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mouse sam library  (Addgene inc)
92
Addgene inc mouse sam library
Mouse Sam Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse sam library/product/Addgene inc
Average 92 stars, based on 1 article reviews
mouse sam library - by Bioz Stars, 2026-04
92/100 stars
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gfp pole3  (Addgene inc)
90
Addgene inc gfp pole3
a, Clonogenic survival of RPE1-hTERT Flag-Cas9 TP53 -/- (WT) and two RPE1-hTERT Flag-Cas9 TP53 -/- APEX2 -/- clones treated with indicated concentrations of ATR inhibitor AZD6738. b, as in a using two CIP2A -/- clones. c, as in a using a <t>POLE3</t> -/- clone. d, as in a using two POLE4 -/- clones. e, as in a using two C16orf72 -/- clones. Data are from three biologically independent experiments.
Gfp Pole3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfp pole3/product/Addgene inc
Average 90 stars, based on 1 article reviews
gfp pole3 - by Bioz Stars, 2026-04
90/100 stars
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theguide itcrisprgenomewide sgrna library ngs analysis kit  (TaKaRa)
94
TaKaRa theguide itcrisprgenomewide sgrna library ngs analysis kit
a, Clonogenic survival of RPE1-hTERT Flag-Cas9 TP53 -/- (WT) and two RPE1-hTERT Flag-Cas9 TP53 -/- APEX2 -/- clones treated with indicated concentrations of ATR inhibitor AZD6738. b, as in a using two CIP2A -/- clones. c, as in a using a <t>POLE3</t> -/- clone. d, as in a using two POLE4 -/- clones. e, as in a using two C16orf72 -/- clones. Data are from three biologically independent experiments.
Theguide Itcrisprgenomewide Sgrna Library Ngs Analysis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/theguide itcrisprgenomewide sgrna library ngs analysis kit/product/TaKaRa
Average 94 stars, based on 1 article reviews
theguide itcrisprgenomewide sgrna library ngs analysis kit - by Bioz Stars, 2026-04
94/100 stars
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sgrna library  (Addgene inc)
94
Addgene inc sgrna library
a, Clonogenic survival of RPE1-hTERT Flag-Cas9 TP53 -/- (WT) and two RPE1-hTERT Flag-Cas9 TP53 -/- APEX2 -/- clones treated with indicated concentrations of ATR inhibitor AZD6738. b, as in a using two CIP2A -/- clones. c, as in a using a <t>POLE3</t> -/- clone. d, as in a using two POLE4 -/- clones. e, as in a using two C16orf72 -/- clones. Data are from three biologically independent experiments.
Sgrna Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrna library/product/Addgene inc
Average 94 stars, based on 1 article reviews
sgrna library - by Bioz Stars, 2026-04
94/100 stars
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cas9 st1  (Addgene inc)
86
Addgene inc cas9 st1
a, Clonogenic survival of RPE1-hTERT Flag-Cas9 TP53 -/- (WT) and two RPE1-hTERT Flag-Cas9 TP53 -/- APEX2 -/- clones treated with indicated concentrations of ATR inhibitor AZD6738. b, as in a using two CIP2A -/- clones. c, as in a using a <t>POLE3</t> -/- clone. d, as in a using two POLE4 -/- clones. e, as in a using two C16orf72 -/- clones. Data are from three biologically independent experiments.
Cas9 St1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cas9 st1/product/Addgene inc
Average 86 stars, based on 1 article reviews
cas9 st1 - by Bioz Stars, 2026-04
86/100 stars
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library of single guide rnas (sgrnas)  (Kuang Lung Shing)
90
Kuang Lung Shing library of single guide rnas (sgrnas)
a, Clonogenic survival of RPE1-hTERT Flag-Cas9 TP53 -/- (WT) and two RPE1-hTERT Flag-Cas9 TP53 -/- APEX2 -/- clones treated with indicated concentrations of ATR inhibitor AZD6738. b, as in a using two CIP2A -/- clones. c, as in a using a <t>POLE3</t> -/- clone. d, as in a using two POLE4 -/- clones. e, as in a using two C16orf72 -/- clones. Data are from three biologically independent experiments.
Library Of Single Guide Rnas (Sgrnas), supplied by Kuang Lung Shing, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
library of single guide rnas (sgrnas) - by Bioz Stars, 2026-04
90/100 stars
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sgrna database  (GenScript corporation)
90
GenScript corporation sgrna database
Troubleshooting table
Sgrna Database, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrna database/product/GenScript corporation
Average 90 stars, based on 1 article reviews
sgrna database - by Bioz Stars, 2026-04
90/100 stars
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sgrna sequence library  (Cellecta Inc)
90
Cellecta Inc sgrna sequence library
Troubleshooting table
Sgrna Sequence Library, supplied by Cellecta Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrna sequence library/product/Cellecta Inc
Average 90 stars, based on 1 article reviews
sgrna sequence library - by Bioz Stars, 2026-04
90/100 stars
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custom mito transporter and salvage pathway sgrna library  (Synthego Inc)
90
Synthego Inc custom mito transporter and salvage pathway sgrna library
Troubleshooting table
Custom Mito Transporter And Salvage Pathway Sgrna Library, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom mito transporter and salvage pathway sgrna library/product/Synthego Inc
Average 90 stars, based on 1 article reviews
custom mito transporter and salvage pathway sgrna library - by Bioz Stars, 2026-04
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human geckov2.0 sgrna lentiviral knockout library  (Shanghai Genechem Ltd)
90
Shanghai Genechem Ltd human geckov2.0 sgrna lentiviral knockout library
Troubleshooting table
Human Geckov2.0 Sgrna Lentiviral Knockout Library, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human geckov2.0 sgrna lentiviral knockout library - by Bioz Stars, 2026-04
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avana library  (Broad Institute Inc)
90
Broad Institute Inc avana library
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Avana Library, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/avana library/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
avana library - by Bioz Stars, 2026-04
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Image Search Results


a, Clonogenic survival of RPE1-hTERT Flag-Cas9 TP53 -/- (WT) and two RPE1-hTERT Flag-Cas9 TP53 -/- APEX2 -/- clones treated with indicated concentrations of ATR inhibitor AZD6738. b, as in a using two CIP2A -/- clones. c, as in a using a POLE3 -/- clone. d, as in a using two POLE4 -/- clones. e, as in a using two C16orf72 -/- clones. Data are from three biologically independent experiments.

Journal: bioRxiv

Article Title: A consensus set of genetic vulnerabilities to ATR inhibition

doi: 10.1101/574533

Figure Lengend Snippet: a, Clonogenic survival of RPE1-hTERT Flag-Cas9 TP53 -/- (WT) and two RPE1-hTERT Flag-Cas9 TP53 -/- APEX2 -/- clones treated with indicated concentrations of ATR inhibitor AZD6738. b, as in a using two CIP2A -/- clones. c, as in a using a POLE3 -/- clone. d, as in a using two POLE4 -/- clones. e, as in a using two C16orf72 -/- clones. Data are from three biologically independent experiments.

Article Snippet: GFP-POLE3 full length and GFP-POLE3 with amino acid residues 113-140 deleted (GFP-POLE3ΔC) were cloned between NheI and AgeI restriction sites of pCW57.1 (Addgene: 41393),

Techniques: Clone Assay

a-c, Immunoblotting to assess loss of protein expression in clonal knockout cell lines. siRNA-mediated knockdown was used to control for antibody specificity in the case of POLE3 and POLE4 antibodies. Antibodies targeting alpha-tubulin (Tubulin) or GAPDH were used as loading controls. Numbers indicate molecular mass in kDa. Asterisks indicate unspecific bands. d, mRNA level analysis of APEX2 after siRNA mediated APEX2 knockdown as assay control and in APEX2 -/- clones. Clone #2 showed mRNA levels similar to parental (WT) cells but has frameshifting mutations (see panel D) and was sensitive to ATR inhibitor treatment (see ).

Journal: bioRxiv

Article Title: A consensus set of genetic vulnerabilities to ATR inhibition

doi: 10.1101/574533

Figure Lengend Snippet: a-c, Immunoblotting to assess loss of protein expression in clonal knockout cell lines. siRNA-mediated knockdown was used to control for antibody specificity in the case of POLE3 and POLE4 antibodies. Antibodies targeting alpha-tubulin (Tubulin) or GAPDH were used as loading controls. Numbers indicate molecular mass in kDa. Asterisks indicate unspecific bands. d, mRNA level analysis of APEX2 after siRNA mediated APEX2 knockdown as assay control and in APEX2 -/- clones. Clone #2 showed mRNA levels similar to parental (WT) cells but has frameshifting mutations (see panel D) and was sensitive to ATR inhibitor treatment (see ).

Article Snippet: GFP-POLE3 full length and GFP-POLE3 with amino acid residues 113-140 deleted (GFP-POLE3ΔC) were cloned between NheI and AgeI restriction sites of pCW57.1 (Addgene: 41393),

Techniques: Western Blot, Expressing, Knock-Out, Clone Assay

a, Whole cell extracts from wild type RPE1-hTERT Flag-Cas9 TP53 -/- (WT) or the indicated POLE4 -/- clones treated with 1 μM camptothecin (CPT) were used for immunoblotting with indicated antibodies. pCHK1 and pRPA refer to phosphorylated proteins; brackets indicate modified amino acid residues. KAP1 served as loading control. b, Whole cell extracts from WT or the indicated POLE3 -/- clone expressing GFP, GFP-POLE3 or GFP-POLE3ΔC were used for immunoblotting with indicated antibodies. GAPDH served as loading control. c, Clonogenic survival of WT or the indicated POLE3 -/- clone expressing GFP, GFP-POLE3 or GFP-POLE3ΔC treated with indicated concentrations of ATR inhibitor AZD6738. Data are from three biologically independent experiments.

Journal: bioRxiv

Article Title: A consensus set of genetic vulnerabilities to ATR inhibition

doi: 10.1101/574533

Figure Lengend Snippet: a, Whole cell extracts from wild type RPE1-hTERT Flag-Cas9 TP53 -/- (WT) or the indicated POLE4 -/- clones treated with 1 μM camptothecin (CPT) were used for immunoblotting with indicated antibodies. pCHK1 and pRPA refer to phosphorylated proteins; brackets indicate modified amino acid residues. KAP1 served as loading control. b, Whole cell extracts from WT or the indicated POLE3 -/- clone expressing GFP, GFP-POLE3 or GFP-POLE3ΔC were used for immunoblotting with indicated antibodies. GAPDH served as loading control. c, Clonogenic survival of WT or the indicated POLE3 -/- clone expressing GFP, GFP-POLE3 or GFP-POLE3ΔC treated with indicated concentrations of ATR inhibitor AZD6738. Data are from three biologically independent experiments.

Article Snippet: GFP-POLE3 full length and GFP-POLE3 with amino acid residues 113-140 deleted (GFP-POLE3ΔC) were cloned between NheI and AgeI restriction sites of pCW57.1 (Addgene: 41393),

Techniques: Clone Assay, Western Blot, Modification, Expressing

Cell survival of RPE1-hTERT Flag-Cas9 TP53 -/- (WT) or the indicated POLE3 -/- clone expressing GFP, GFP-POLE3 or GFP-POLE3ΔC treated with indicated concentrations of ATR inhibitor (AZD6738) was determined by monitoring growth in an Incucyte instrument. Data are from three biologically independent experiments.

Journal: bioRxiv

Article Title: A consensus set of genetic vulnerabilities to ATR inhibition

doi: 10.1101/574533

Figure Lengend Snippet: Cell survival of RPE1-hTERT Flag-Cas9 TP53 -/- (WT) or the indicated POLE3 -/- clone expressing GFP, GFP-POLE3 or GFP-POLE3ΔC treated with indicated concentrations of ATR inhibitor (AZD6738) was determined by monitoring growth in an Incucyte instrument. Data are from three biologically independent experiments.

Article Snippet: GFP-POLE3 full length and GFP-POLE3 with amino acid residues 113-140 deleted (GFP-POLE3ΔC) were cloned between NheI and AgeI restriction sites of pCW57.1 (Addgene: 41393),

Techniques: Expressing

Troubleshooting table

Journal: Nature protocols

Article Title: Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

doi: 10.1038/s41596-021-00669-0

Figure Lengend Snippet: Troubleshooting table

Article Snippet: To identify an sgRNA target sequence using the GenScript sgRNA database ( https://www.genscript.com/gRNA-database.html ) or other webtools, input the gene name, symbol or ID and search for human SpCas9 gRNA sequences.

Techniques: Negative Control, Ligation, Transformation Assay, Plasmid Preparation, Selection, Passaging, Clone Assay, Concentration Assay, Sequencing